NanoBodyBuilder2

NanoBodyBuilder2 predicts all-atom 3D structures for single-chain nanobody (VHH) heavy-chain sequences, with a focus on accurate CDR loop conformations, including CDR3. The API accepts nanobody heavy-chain sequences (``H``) up to 2048 residues and returns refined PDB models produced via a deep learning backbone followed by physics-based energy minimisation. NanoBodyBuilder2 runs on CPU-only nodes (2 vCPUs, 8 GB RAM) and is suitable for high-throughput nanobody repertoire analysis, developability assessment, and structure-guided design workflows.