Soluble ProteinMPNN

Design soluble protein sequences for complex and membrane-derived folds from fixed backbones using a ProteinMPNN variant retrained on soluble structures. The service samples sequences that match the input backbone while reducing surface hydrophobic residues, and supports per-residue constraints, amino acid sampling biases, and global or per-residue transmembrane labeling controls. Typical use cases include designing soluble analogs of GPCR-, claudin-, and rhomboid-like folds for structure-function studies and screening-compatible scaffolds.